Isolation, Identification and Serotyping Uropathogenic Escherchia Coli Siderophore Manufacturer of Hospital Patients and Health Care Centers in Bandare Anzali

─ Acute urinary tract infections consist of upper urinary tract infections, including pyelonephritis, prostatitis, intra-kidney abscesses, and around-kidney abscesses, and lower urinary tract infections, including urethra infection and bladder infection. These infections can independently or in combination result in irritation in various parts. They may appear as asymptomatic or as one of the clinical syndromes. Bladder and urethra infections are as superficial (mucosal) infections but prostatitis and pyelonephritis and the accumulation of pus in the kidney shows the tissue invasion. In microbiological terms, there is urinary infection when pathogenic microorganisms are found in urine, urethral, bladder, kidney, or prostate. In most cases, the growth of more than 105 microorganisms per milliliter of the samples in the middle of urine indicates the infection, but in symptomatic cases, 102-104 bacteria per milliliter of urine also suggest the infection. The symptoms of dysuria, urgent and frequent urination not associated with significant bacteriuria is called urethral syndrome. This study aimed to determine the local incidence of urinary tract infection, to identify UPEC strains, serotyping, and antimicrobial resistance, and to investigate siderophore and hemolysin and their correlation with the ability to cause infection in Escherichia coli strains isolated from these patients. In order to separate and identify UPEC bacterial factors of urinary tract infection and to determine age and sex distribution of the ones infected in a prospective study, 200 samples taken from the middle of urine were analyzed with simple random sampling. The test of sensitivity to various antibiotics was done with Kirby-Bauer method, that is, disc diffusion in agar on 21 UPEC isolates separated from these patients in the 1-86 age range, and serotyping with agglutination method on a slide and hemolysin production was evaluated on blood agar with 5% sheep blood. Also, siderophore production was studied in isolates. The whole results were analyzed by using the SPSS software. The majority of urinary pathogen UPEC isolates belonged to the serotypes O114, O26, O64, O55, O15, O44, O25, O20, O1, O126, and O157. The majority of isolates had the highest resistance to the antibiotics ampicillin, cotrimoxazole, cefixime, and cephalexin and the highest sensitivity to nitrofurantoin, amikacin, gentamicin, ciprofloxacin, and tetracycline. The present study showed that O25 serotype includes the largest number of isolates and other serotypes, in order of frequency, were O20, O44, O1, O55, O114, O126, O15, O64, O26, and O157.